Asian Journal of Pharmacy and Technology

Tabassum H. Aaraf ¹, Hasumati A. Raj ¹, Vineet C. Jain ¹, Vishnu Sutariya ²

Affiliations
1 Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, IN
2 Shree Dhanvantary Pharmaceutical Analysis & Research Centre, Kim, Surat, Gujarat, IN

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Metronidazole Benzoate and related impurity in bulk and pharmaceutical formulation by ratio derivative simultaneous equation method. The method is based on dividing the spectrum for a mixture into the standard spectra for each of the analysis and to obtain a spectrum that is independent of the analyte concentration used as a divisor. In this method for MND, MNI and BA the linearity range 2.0-10 μg/ml were divided by the divisor 2.0 μg/ml of MTZ. The use of standardized spectra as divisors minimizes experimental errors. Ratio spectra derivative permits the use of the wavelengths corresponding to maximum or minimum and also the use of the distance between consecutive maximum and minimum. For that the divided spectra was converted to second derivative using DL value 16 and SF value 100. The Metronidazole shows max absorbance at 270.00 nm, 2- Methyl-5Nitroimidazole show max absorbance at 257.42 nm and Benzoic Acid shows max. absorbance at 245.61 nm. The method was found to be linear (r2>0.997) in the range of 2.0-10 μg/ml. The limit of determination was 0.049 μg/ml and 0.026 μg/ml and0.038 μg/ml for Metronidazole, 2-Methyl-5-Nitroimidazole and Benzoic Acid, respectively. The limit of quantification was 0.150 μg/ml and 0.079 μg/ml and 0.115 μg/ml for Metronidazole, 2-Methyl-5-Nitroimidazole and Benzoic Acid, respectively. The accuracy of this method was evaluated by recovery studies and good recovery result was obtained 100%. The method was successfully applied for simultaneous determination of Metronidazole, 2-Methyl-5-Nitroimidazole and Benzoic Acid.

Keywords

Metronidazole Benzoate, Metronidazole, 2-Methyl-5-Nitroimidazole, Benzoic Acid, Ratio Derivative Simultaneous Equation Method.

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Grishma S. Trivedi ¹, Hasumati A. Raj ¹, Vineet C. Jain ¹

Affiliations
1 Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, IN

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Pravastatin and Valsartan in synthetic mixture using first order derivative zero-crossing method. Pravastatin showed zero crossing point at 262.40 nm while Valsartan showed zero crossing point at 248.20 nm. The dA/dλ was measured at 248.20 nm for Pravastatin and 262.80 nm for Valsartan and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.999) in the range of 2-10μg/ml for Pravastatin at 248.20 nm. The linear correlation was obtained (r2>0.9998) in the range of 8-40μg/ml for Valsartan at 262.40 nm. The limit of determination was 0.054μg/ml and 0.024μg/ml for Pravastatin and Valsartan, respectively. The limit of quantification was 0.166μg/ml and 0.074μg/ml for Pravastatin and Valsartan, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Pravastatin and Valsartan in binary mixture.

Keywords

Pravastatin, Valsartan, First Derivative Method, Spectroscopic Method.

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Monika A. Rana ¹, Hasumati A. Raj ¹

Affiliations
1 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim Surat, Gujarat, IN

Abstract

A simple, accurate and precise chromatography method was developed for Levosulpiride and 2-methoxy 5- sulfamoylbenzoyl benzoic acid methyl ester in synthetic mixture using RP-HPLC Method. In this chromatography method, both drug show its peak at 232 nm. Both the drugs show linearity in a concentration range of 10-50 μg/ml at their respective λmax. Optimize Mobile Phase was Water : Methanol : Acetonitrile (70 : 15 : 15). The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.14μg/ml and 0.19μg/ml for Levosulpiride and 2-methoxy 5- sulfamoylbenzoyl benzoic acid methyl ester, respectively. The limit of quantification was 0.42μg/ml and 0.59μg/ml for Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester, respectively. Recovery of Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester were found to be 100.16 % and 99.15 % respectively confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.

Keywords

Levosulpiride, 2-Methoxy 5-Sulfamoylbenzoyl Benzoic Acid Methyl Ester, RP-HPLC Method.

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